1BPE
CRYSTAL STRUCTURE OF RAT DNA POLYMERASE BETA; EVIDENCE FOR A COMMON POLYMERASE MECHANISM
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.319, 68.208, 75.038 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.900 * |
| R-factor | 0.199 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.000 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.900 * |
| Rmerge | 0.067 * |
| Total number of observations | 12400 * |
| Number of reflections | 4069 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG3350 | 7.5 (%(w/v)) | |
| 2 | 1 | drop | Tris | 100 (mM) | |
| 3 | 1 | drop | 15 (mM) | ||
| 4 | 1 | drop | 39-kD pol beta | 15 (mg/ml) | |
| 5 | 1 | drop | HEPES | 100 (mM) | |
| 6 | 1 | drop | 10 (mM) | ||
| 7 | 1 | reservoir | PEG3350 | 25 (%) | |
| 8 | 1 | reservoir | HEPES | 100 (mM) | |
| 9 | 1 | reservoir | 15 (mM) |
