1BOF
GI-ALPHA-1 BOUND TO GDP AND MAGNESIUM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-06 |
| Detector | ADSC QUANTUM 1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 121.747, 121.747, 68.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.23 * |
| Rwork | 0.227 |
| R-free | 0.26700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gdd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.050 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.039 | 0.103 |
| Total number of observations | 91667 * | |
| Number of reflections | 25367 | |
| <I/σ(I)> | 27.5 | 13.1 |
| Completeness [%] | 97.3 | 96.1 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 1.4 (M) | |
| 3 | 1 | drop | EPPS | 25 (mM) | |
| 4 | 1 | drop | 5 (mM) | or 10mM | |
| 5 | 1 | drop | GTPgammaS | 0.5 (mM) | |
| 6 | 1 | drop | dithiothreitol | 2.5 (mM) | |
| 7 | 1 | drop | EDTA | 10 (mM) | |
| 8 | 1 | reservoir | 100 (mM) | ||
| 9 | 1 | reservoir | potassium phosphate | 1.45-1.65 (M) |
