1BOA
HUMAN METHIONINE AMINOPEPTIDASE 2 COMPLEXED WITH ANGIOGENESIS INHIBITOR FUMAGILLIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 1998-06 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 90.700, 99.580, 101.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.800 |
R-factor | 0.194 * |
Rwork | 0.193 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xgs |
RMSD bond length | 0.013 |
RMSD bond angle | 0.028 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.600 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.078 | 0.149 |
Total number of observations | 158945 * | |
Number of reflections | 40944 | |
<I/σ(I)> | 13.4 | 6.8 |
Completeness [%] | 94.7 | 99.4 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.2 * | 4 * | pH 5.4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | drop | glycerol | 10 (%) | |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | reservoir | t-butanol | 15-30 (%) | |
6 | 1 | reservoir | sodium citrate | 33-100 (mM) |