1BNZ
SSO7D HYPERTHERMOPHILE PROTEIN/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.600, 50.770, 42.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.26800 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Phasing software | PHASIT |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | |
| High resolution limit [Å] | 1.900 | 2.000 * |
| Rmerge | 0.075 | |
| Number of reflections | 7607 | |
| Completeness [%] | 94.1 | 83 * |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Sso7d | 1.3 (mM) | |
| 2 | 1 | drop | DNA duplex | 1.3 (mM) | |
| 3 | 1 | drop | Tris-Cl | 2 (mM) | |
| 4 | 1 | drop | PEG400 | 2.6 (%) | |
| 5 | 1 | reservoir | PEG400 | 15 (%) |
