1BNG

BARNASE S85C/H102C DISULFIDE MUTANT

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993
Detector	MAR scanner 300 mm plate
Spacegroup name	P 32
Unit cell lengths	59.450, 59.450, 81.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.17
RMSD bond length	0.010
RMSD bond angle	0.033
Data reduction software	MOSFLM
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	15.500 *
High resolution limit [Å]	2.100 *
Rmerge	0.097
Total number of observations	39029 *
Number of reflections	18973
Completeness [%]	93.8
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-20 (mg/ml)
2	1	drop		10 (mM)
3	1	drop	Tris-HCl	25 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop	dithiothreitol	1 (mM)
6	1	reservoir	PEG8000	35 (%)
7	1	reservoir	sodium acetate	50 (mM)
8	1	reservoir	sodium cacodylate	50 (mM)