1BNF
BARNASE T70C/S92C DISULFIDE MUTANT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS |
| Synchrotron site | SRS |
| Detector technology | IMAGE PLATE |
| Collection date | 1993 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 32 |
| Unit cell lengths | 59.140, 59.140, 81.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.173 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.042 |
| Data reduction software | MOSFLM |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.300 * |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.122 |
| Total number of observations | 50660 * |
| Number of reflections | 20982 |
| Completeness [%] | 97.2 |
| Redundancy | 2.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-20 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 25 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 35-40 (%) | |
| 7 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 8 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 9 | 1 | reservoir | sodium phosphate | 5 (mM) |
