1BNC

THREE-DIMENSIONAL STRUCTURE OF THE BIOTIN CARBOXYLASE SUBUNIT OF ACETYL-COA CARBOXYLASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	61.900, 96.100, 180.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.183
RMSD bond length	0.013
RMSD bond angle	2.300
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.039 *
Total number of observations	93923 *
Number of reflections	41566 *
Completeness [%]	75.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7 *	4 *	Waldrop, G., (1994) J. Mol. Biol, 235, 367. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	potassium phosphate	10 (mM)
2	1	2	EDTA	1 (mM)
3	1	2	DTT	2 (mM)
4	1	2		1 (mM)
5	1	1	protein	10 (mg/ml)