1BMZ

HUMAN TRANSTHYRETIN (PREALBUMIN)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-05-15
Detector	MACSCIENCE
Spacegroup name	P 21 21 2
Unit cell lengths	42.850, 85.800, 64.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.183
Rwork	0.183
R-free	0.24400
Structure solution method	OTHER
RMSD bond length	0.015
RMSD bond angle	2.013
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.052	0.117
Total number of observations	64327 *
Number of reflections	16218
<I/σ(I)>	13.9
Completeness [%]	96.7	84
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4		pH 7.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop		100 (mM)
3	1	drop	phosphate	100 (mM)
4	1	drop	ammonium sulfate	1 (M)
5	1	reservoir	ammonium sulfate	2 (M)