1BMS

CRYSTAL STRUCTURE OF MS2 CAPSIDS WITH MUTATIONS IN THE SUBUNIT FG LOOP

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	0.87, 0.93, 0.95
Spacegroup name	H 3 2
Unit cell lengths	288.000, 288.000, 653.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.218
Rwork	0.218
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.590 *
High resolution limit [Å]	2.670 *	2.670 *
Rmerge	0.090	0.166 *
Number of reflections	140876	1468 *
Completeness [%]		7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	37 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MS2	1.0 (%(w/v))
2	1	drop	sodium phosphate	0.2 (M)
3	1	drop	PEG6000	1.5 (%(w/v))
4	1	drop		0.02 (%(w/v))
5	1	reservoir	sodium phospahte	0.4 (M)