1BMF
BOVINE MITOCHONDRIAL F1-ATPASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 269 |
Detector technology | IMAGE PLATE |
Collection date | 1993-09-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 284.220, 107.760, 139.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.850 |
R-factor | 0.172 * |
R-free | 0.25400 * |
Starting model (for MR) | CCP4 |
RMSD bond length | 0.011 |
RMSD bond angle | 0.030 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | MULTIPLE (ISOMORPHOUS REPLACEMENT) |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.098 | 0.190 |
Number of reflections | 96689 | |
Completeness [%] | 97.5 | 92 |
Redundancy | 5.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 8.2 | 22-24 * | pH 8.2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | Tris-HCl | 50 (mM) | |
10 | 1 | 1 | AMP-PNP | 0.25 (M) | * |
11 | 1 | 1 | ADP | 0.005 (M) | * |
12 | 1 | 2 | magnesium chloride | 8 (mM) | |
13 | 1 | 2 | PEG6000 | 14 (%(w/v)) | |
14 | 1 | 2 | double the amount of * | ||
2 | 1 | 1 | PEG6000 | 9 (%(w/v)) | |
3 | 1 | 1 | sodium chloride | 200 (mM) | * |
4 | 1 | 1 | EDTA | 1 (mM) | * |
5 | 1 | 1 | sodium azide | 0.02 (%(w/v)) | * |
6 | 1 | 1 | 2-mercaptoethanol | 5 (mM) | |
7 | 1 | 1 | PMSF | 0.001 (%) | |
8 | 1 | 1 | dithiothreitol | 5 (mM) | * |
9 | 1 | 1 | magnesium sulphate | 20 (mM) |