1BMF
BOVINE MITOCHONDRIAL F1-ATPASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 269 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 284.220, 107.760, 139.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.850 |
| R-factor | 0.172 * |
| R-free | 0.25400 * |
| Starting model (for MR) | CCP4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.030 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MULTIPLE (ISOMORPHOUS REPLACEMENT) |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.098 | 0.190 |
| Number of reflections | 96689 | |
| Completeness [%] | 97.5 | 92 |
| Redundancy | 5.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 8.2 | 22-24 * | pH 8.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 10 | 1 | 1 | AMP-PNP | 0.25 (M) | * |
| 11 | 1 | 1 | ADP | 0.005 (M) | * |
| 12 | 1 | 2 | magnesium chloride | 8 (mM) | |
| 13 | 1 | 2 | PEG6000 | 14 (%(w/v)) | |
| 14 | 1 | 2 | double the amount of * | ||
| 2 | 1 | 1 | PEG6000 | 9 (%(w/v)) | |
| 3 | 1 | 1 | sodium chloride | 200 (mM) | * |
| 4 | 1 | 1 | EDTA | 1 (mM) | * |
| 5 | 1 | 1 | sodium azide | 0.02 (%(w/v)) | * |
| 6 | 1 | 1 | 2-mercaptoethanol | 5 (mM) | |
| 7 | 1 | 1 | PMSF | 0.001 (%) | |
| 8 | 1 | 1 | dithiothreitol | 5 (mM) | * |
| 9 | 1 | 1 | magnesium sulphate | 20 (mM) |
