1BMC
STRUCTURE OF A ZINC METALLO-BETA-LACTAMASE FROM BACILLUS CEREUS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-07 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.790, 61.980, 69.970 |
| Unit cell angles | 90.00, 93.67, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.33000 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.470 * |
| Data reduction software | MADNES |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.030 |
| Total number of observations | 11206 * |
| Number of reflections | 6911 |
| Completeness [%] | 86.0 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | cacodylate | 10 (mM) | |
| 3 | 1 | drop | 0.1 (mM) | ||
| 4 | 1 | drop | DTT | 0.050 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 25 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 18 (%(w/v)) |
