1BM8
DNA-BINDING DOMAIN OF MBP1
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 0.96, 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 43.350, 43.350, 124.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.710 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.26100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.710 |
| Rmerge | 0.052 | |
| Total number of observations | 68232 * | |
| Number of reflections | 12811 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 93.6 | 34 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 16 * | drop contains equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 300 (mM) | ||
| 3 | 1 | drop | sodium phosphate | 50 (mM) | |
| 4 | 1 | reservoir | sodium citrate | 70 (%sat) | |
| 5 | 1 | reservoir | imidazole | 100 (mM) |
