1BM1

CRYSTAL STRUCTURE OF BACTERIORHODOPSIN IN THE LIGHT-ADAPTED STATE

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	285
Detector technology	IMAGE PLATE
Collection date	1996-11-14
Detector	FUJI
Spacegroup name	P 6 2 2
Unit cell lengths	104.700, 104.700, 114.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 3.500
R-factor	0.269 *
Rwork	0.260
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2brd
RMSD bond length	0.009
RMSD bond angle	20.076 *
Data reduction software	WEIS
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.650
High resolution limit [Å]	3.500	3.500
Rmerge	0.103	0.159
Number of reflections	4000
Completeness [%]	80.2	45.2
Redundancy	7.4	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2	278 *	pH 5.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.0 (M)
2	1	reservoir	sodium citrate	0.08 (M)