1BLH

STRUCTURE OF A PHOSPHONATE-INHIBITED BETA-LACTAMASE. AN ANALOG OF THE TETRAHEDRAL TRANSITION STATE(SLASH)INTERMEDIATE OF BETA-LACTAM HYDROLYSIS

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	53.960, 94.200, 138.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.166
RMSD bond length	0.019
RMSD bond angle	0.040
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Total number of observations	46421 *
Number of reflections	14163 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8 *		referred to 'Herzberg, O.', (1987) Science, 236, 694-701 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	78 (%sat)
2	1	1		0.3 (M)
3	1	1		100 (mM)