1BLF
STRUCTURE OF DIFERRIC BOVINE LACTOFERRIN AT 2.8 ANGSTROMS RESOLUTION
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-05 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 138.860, 87.010, 73.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.232 |
| Rwork | 0.217 |
| R-free | 0.28600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN LACTOFERRIN |
| RMSD bond length | 0.011 |
| RMSD bond angle | 17.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.048 | 0.310 |
| Total number of observations | 81067 * | |
| Number of reflections | 22017 | |
| <I/σ(I)> | 28 | 4 |
| Completeness [%] | 97.0 | 95.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.7 | 4 * | 25 MM TRIS/HCL, PH 7.7 8% (V/V) MPD 6.5% (V/V) ETHANOL DIALYSED AGAINST A 200 MG/ML SOLUTION OF PROTEIN AT 277 K., dialaysis |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | bLf | 200 (mg/ml) | |
| 2 | 1 | 2 | Tris-HCl | 25 (mM) | |
| 3 | 1 | 2 | MPD | 8 (%(v/v)) | |
| 4 | 1 | 2 | methanol | 6.5 (%(v/v)) |
