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1BLE

PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM

Experimental procedure
Source typeSYNCHROTRON
Source detailsSRS BEAMLINE PX9.6
Synchrotron siteSRS
BeamlinePX9.6
Temperature [K]293
Detector technologyIMAGE PLATE
Collection date1995-12-02
DetectorMARRESEARCH
Spacegroup nameP 43 3 2
Unit cell lengths106.900, 106.900, 106.900
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.900
R-factor0.185
Rwork0.185
R-free0.26300
Structure solution methodMIRAS
RMSD bond length0.014
RMSD bond angle25.200

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Data reduction softwareMOSFLM
Data scaling softwareCCP4
Phasing softwareX-PLOR (3.1)
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0003.000
High resolution limit [Å]2.9002.900
Rmerge0.0540.288
Number of reflections4889
Completeness [%]97.796.7
Redundancy3.53.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

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7

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20

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drop solution was mixed with an equal volume of reservoir solution

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Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein18-24 (mg/ml)
21dropHEPES5 (mM)
31reservoirsodium formate3.2-3.8 (M)
41reservoirTris-HCl0.1 (M)

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