1BLC

INHIBITION OF BETA-LACTAMASE BY CLAVULANATE: TRAPPED INTERMEDIATES IN CRYOCRYSTALLOGRAPHIC STUDIES

Experimental procedure

Temperature [K]	120
Spacegroup name	I 2 2 2
Unit cell lengths	54.100, 88.000, 141.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.179
RMSD bond length	0.022
RMSD bond angle	0.041
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	3.720 *
High resolution limit [Å]	2.050 *	2.050 *
Rmerge	0.046 *	0.072 *
Total number of observations	46421 *
Completeness [%]	97.0 *	96 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8 *		referred to 'Moult, J.', (1985) Biochem.J., 225, 167. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	60-75 (%sat)
2	1	1	protein	10-20 (mg/ml)