1BLB

CLOSE PACKING OF AN OLIGOMERIC EYE LENS BETA-CRYSTALLIN INDUCES LOSS OF SYMMETRY AND ORDERING OF SEQUENCE EXTENSIONS

Experimental procedure

Spacegroup name	C 2 2 2
Unit cell lengths	154.710, 165.900, 78.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.600 *
R-factor	0.205 *
RMSD bond length	0.013 *
RMSD bond angle	3.100 *
Phasing software	X-PLOR
Refinement software	RESTRAIN

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (%(w/w))
2	1	drop	Tris-acetate	0.05 (M)
3	1	drop	sodium azide	0.02 (%(w/v))
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	MPD	35 (%(w/v))
6	1	reservoir	Tris-acetate	0.05 (M)