1BL5
ISOCITRATE DEHYDROGENASE FROM E. COLI SINGLE TURNOVER LAUE STRUCTURE OF RATE-LIMITED PRODUCT COMPLEX, 10 MSEC TIME RESOLUTION
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 274 |
Detector technology | IMAGE PLATE |
Collection date | 1995-01-01 |
Detector | FUJI |
Wavelength(s) | 0.7, 1.7 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 106.100, 106.100, 151.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.27100 |
Structure solution method | OTHER |
RMSD bond length | 0.017 |
RMSD bond angle | 2.800 |
Data reduction software | LaueView |
Data scaling software | LaueView |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.500 | 2.400 |
Rmerge | 0.102 | 0.237 |
Total number of observations | 360784 * | |
Number of reflections | 21751 | |
<I/σ(I)> | 17.2 | 7.5 |
Completeness [%] | 90.8 | 83.8 |
Redundancy | 12.9 | 16.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5.4 * | Hurley, J.H., (1989) Proc.Nat.Acad.Sci.USA, 86, 8635. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 28 (mg/ml) | |
2 | 1 | 1 | ammonium sulfate | 34 (%sat) | |
3 | 1 | 1 | 100 (mM) | ||
4 | 1 | 1 | 35 (mM) | ||
5 | 1 | 1 | citric acid | 9 (mM) | |
6 | 1 | 1 | dithiothreitol | 0.2 (mM) |