1BL5

ISOCITRATE DEHYDROGENASE FROM E. COLI SINGLE TURNOVER LAUE STRUCTURE OF RATE-LIMITED PRODUCT COMPLEX, 10 MSEC TIME RESOLUTION

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	274
Detector technology	IMAGE PLATE
Collection date	1995-01-01
Detector	FUJI
Wavelength(s)	0.7, 1.7
Spacegroup name	P 43 21 2
Unit cell lengths	106.100, 106.100, 151.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.222
Rwork	0.222
R-free	0.27100
Structure solution method	OTHER
RMSD bond length	0.017
RMSD bond angle	2.800
Data reduction software	LaueView
Data scaling software	LaueView
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.500	2.400
Rmerge	0.102	0.237
Total number of observations	360784 *
Number of reflections	21751
<I/σ(I)>	17.2	7.5
Completeness [%]	90.8	83.8
Redundancy	12.9	16.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.4 *		Hurley, J.H., (1989) Proc.Nat.Acad.Sci.USA, 86, 8635. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	28 (mg/ml)
2	1	1	ammonium sulfate	34 (%sat)
3	1	1		100 (mM)
4	1	1		35 (mM)
5	1	1	citric acid	9 (mM)
6	1	1	dithiothreitol	0.2 (mM)