1BL0
MULTIPLE ANTIBIOTIC RESISTANCE PROTEIN (MARA)/DNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 96 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-01 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 47.100, 47.100, 298.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.30300 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 21.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.067 * | 0.770 * |
Total number of observations | 121112 * | |
Number of reflections | 16074 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 7.5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | micro seeding * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium CACODYLATE | ||
2 | 1 | 1 | calcium ACETATE | ||
3 | 1 | 1 | PEG 8000 | ||
4 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 12 (%) | |
2 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
3 | 1 | reservoir | calcium acetate | 100 (mM) |