1BKR
CALPONIN HOMOLOGY (CH) DOMAIN FROM HUMAN BETA-SPECTRIN AT 1.1 ANGSTROM RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.650, 53.953, 32.354 |
| Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.100 |
| R-factor | 0.141 |
| R-free | 0.18700 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.110 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.044 | 0.419 |
| Total number of observations | 199306 * | |
| Number of reflections | 42236 | |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.6 | 20 * | Djinovic, C., (1997) Nat. Struct. Biol., 4, 175. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CH domain | 10 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 20 (mM) | |
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 7 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 8 | 1 | reservoir | PEG8000 | 30 (%(w/v)) |
