1BKN
CRYSTAL STRUCTURE OF AN N-TERMINAL 40KD FRAGMENT OF E. COLI DNA MISMATCH REPAIR PROTEIN MUTL
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 87.200, 93.200, 221.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.30000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 27.000 * |
| Data reduction software | HKL |
| Data scaling software | CCP4 |
| Phasing software | MAMA |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.074 * | 0.696 * |
| Number of reflections | 23519 | |
| <I/σ(I)> | 8.3 | 1.1 |
| Completeness [%] | 96.5 | 84.6 |
| Redundancy | 4.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.6 | 20 * | pH 6.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | bis-Tris propane | 100 (mM) | |
| 2 | 1 | reservoir | magnesium acetate | 100 (mM) | |
| 3 | 1 | reservoir | magnesium sulfate | 100 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 20 (%) |
