1BKF
FK506 BINDING PROTEIN FKBP MUTANT R42K/H87V COMPLEX WITH IMMUNOSUPPRESSANT FK506
Experimental procedure
| Detector | SIEMENS |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 58.360, 58.360, 56.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.600 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.980 * |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 6.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.035 |
| Number of reflections | 11511 |
| <I/σ(I)> | 3 |
| Completeness [%] | 88.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.9 * | van Duyne, G. D., (1991) Science, 252, 839. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 1.7 (M) | ||
| 3 | 1 | drop | phosphate | 0.1 (M) |
