1BKD
COMPLEX OF HUMAN H-RAS WITH HUMAN SOS-1
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02 |
| Detector | BRANDEIS |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 142.700, 142.700, 207.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.28100 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.260 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.530 | 0.287 |
| Total number of observations | 283114 * | |
| Number of reflections | 26562 | |
| <I/σ(I)> | 34 | 5.9 |
| Completeness [%] | 99.3 | 99.9 * |
| Redundancy | 5.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 4 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | sodium formate | 2.7-3.2 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) |
