1BK5
KARYOPHERIN ALPHA FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-02 |
Detector | BRANDEIS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 157.980, 74.140, 84.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.500 - 2.200 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.27600 |
Structure solution method | MIR (MULTIPLE ISOMORPHOUS REPLACEMENT) |
RMSD bond length | 0.007 |
RMSD bond angle | 17.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (0.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.500 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.065 * | 0.299 * |
Total number of observations | 525332 * | |
Number of reflections | 48255 | |
<I/σ(I)> | 13.6 | 2.8 |
Completeness [%] | 95.0 | 95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 4 * | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 18-20 (%) | |
2 | 1 | reservoir | sodium acetate | 200 (mM) | |
3 | 1 | reservoir | HEPES | 100 (mM) | |
4 | 1 | reservoir | 20 (mM) |