1BK4

CRYSTAL STRUCTURE OF RABBIT LIVER FRUCTOSE-1,6-BISPHOSPHATASE AT 2.3 ANGSTROM RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	285
Detector technology	AREA DETECTOR
Collection date	1990-01-15
Detector	UCSD MARK III
Spacegroup name	I 2 2 2
Unit cell lengths	73.790, 80.050, 131.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.177
Rwork	0.177
Structure solution method	MIRAS
RMSD bond length	0.012
RMSD bond angle	1.700
Data reduction software	UCSD-system
Data scaling software	UCSD-system
Phasing software	PHASES
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.200
Rmerge	0.057
Total number of observations	67887 *
Number of reflections	16921
<I/σ(I)>	12.8
Completeness [%]	93.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4		10-15% PEG 4000, 50-200 MM A.S. IN 25 MM TRIS, pH 7.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	10-15 (%)
2	1	reservoir	Tris-HCl	25 (mM)	pH7.4
3	1	drop	protein	7-8 (mg/ml)
4	1	drop	ammonium sulfate	50-200 (mM)