1BK1

ENDO-1,4-BETA-XYLANASE C

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-07-04
Detector	FUJI
Spacegroup name	P 43 21 2
Unit cell lengths	62.065, 62.065, 113.291
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.194
Rwork	0.194
R-free	0.25900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRICHODERMA REESEI XYNI PDB ENTRY 1XYN
RMSD bond length	0.010
RMSD bond angle	23.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.700	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.073	0.397
Total number of observations	134544 *
Number of reflections	15428
<I/σ(I)>	10.3
Completeness [%]	98.4	97.2
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	3.5 *	25 *	0.02ml of protein solution was mixed with 0.01ml of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-8 (mg/ml)
2	1	drop	acetic acid	5 (mM)
3	1	reservoir	HEPES-NaOH	100 (mM)
4	1	reservoir	sodium sulphate	1.5 (M)