1BJN

STRUCTURE OF PHOSPHOSERINE AMINOTRANSFERASE FROM ESCHERICHIA COLI

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	68.740, 94.740, 133.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.500 - 2.300
R-factor	0.172 *
Rwork	0.175
R-free	0.20100
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	24.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.500	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.083 *
Number of reflections	38831
<I/σ(I)>	14.7	3.3
Completeness [%]	98.2	99.9
Redundancy	4.5	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		4-7 *	pH7.2 in the drop and pH5.6 in the reservoir *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000
2	1	reservoir	sodium acetate