1BJK
FERREDOXIN:NADP+ REDUCTASE MUTANT WITH ARG 264 REPLACED BY GLU (R264E)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 65 |
| Unit cell lengths | 88.090, 88.090, 97.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.000 - 2.300 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1que |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.170 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.580 | 2.430 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.118 | 0.400 |
| Number of reflections | 18847 | |
| <I/σ(I)> | 6.1 | 1.8 |
| Completeness [%] | 99.1 | 94.4 |
| Redundancy | 8.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | 20 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.3 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 4 (mM) | |
| 3 | 1 | drop | beta-octylglucoside | 1 (%(w/v)) | |
| 4 | 1 | drop | PEG6000 | 10.8 (%(w/v)) | |
| 5 | 1 | drop | ammonium sulfate | 8 (mM) | |
| 6 | 1 | drop | MES | 0.04 (M) | |
| 7 | 1 | reservoir | PEG6000 | 17 (%(w/v)) | |
| 8 | 1 | reservoir | ammonium sulfate | 20 (mM) | |
| 9 | 1 | reservoir | MES | 0.1 (M) |
