1BJE
H64T VARIANT OF MYOGLOBIN (HORSE HEART) RECOMBINANT WILD-TYPE COMPLEXED WITH AZIDE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10-10 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.019, 28.812, 35.840 |
| Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| Rwork | 0.178 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.036 |
| Data reduction software | OSCILL |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Rmerge | 0.105 |
| Total number of observations | 19842 * |
| Number of reflections | 8046 |
| Completeness [%] | 64.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.4 * | 25 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 65 (%sat) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | 25 (mM) | ||
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 70 (%sat) | |
| 7 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 8 | 1 | reservoir | EDTA | 1 (mM) |
