1BJA
ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1994-06 |
Detector | RIGAKU |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 46.700, 46.700, 139.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.190 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.29100 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 24.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.300 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.054 * | 0.081 |
Total number of observations | 66237 * | |
Number of reflections | 9394 | |
<I/σ(I)> | 11 | 2.5 |
Completeness [%] | 98.5 | 80 |
Redundancy | 7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 22 * | Finnin, M.S., (1993) J. Mol. Biol., 232, 301. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 60 (%sat) |