1BIH

CRYSTAL STRUCTURE OF THE INSECT IMMUNE PROTEIN HEMOLIN: A NEW DOMAIN ARRANGEMENT WITH IMPLICATIONS FOR HOMOPHILIC ADHESION

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1997-01
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	85.000, 90.300, 143.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.100
R-factor	0.218
Rwork	0.218
R-free	0.26400
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	30.700 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MLPHARE
Refinement software	X-PLOR (3.857)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	3.300
High resolution limit [Å]	3.100	3.100
Rmerge	0.077	0.246 *
Number of reflections	19430	1241 *
<I/σ(I)>	9.4	3.1
Completeness [%]	92.0	56
Redundancy	2.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.1		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	6 (mg/ml)
2	1	1	phosphate	50 (mM)
3	1	1		0.15 (M)
4	1	1	sodium potassium phosphate	1.8 (M)