1BHZ

LOW TEMPERATURE MIDDLE RESOLUTION STRUCTURE OF HEN EGG WHITE LYSOZYME FROM MASC DATA

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW21B
Synchrotron site	LURE
Beamline	DW21B
Temperature [K]	124
Detector technology	IMAGE PLATE
Collection date	1996-11
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	78.680, 78.680, 37.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.900
R-factor	0.315
Rwork	0.315
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT, RIGID BODY REFINEMENT
Starting model (for MR)	1lse
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.500	4.000
High resolution limit [Å]	3.910	3.910
Rmerge	0.028	0.028
Total number of observations	10257 *
Number of reflections	1092
<I/σ(I)>	18.3	46.7
Completeness [%]	84.7	78
Redundancy	9.4	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN WAS CRYSTALLIZED FROM SODIUM CHLORIDE SOLUTIONS