1BHY
LOW TEMPERATURE MIDDLE RESOLUTION STRUCTURE OF P64K FROM MASC DATA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 124 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 140.620, 140.620, 77.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 4.180 |
| R-factor | 0.338 |
| Rwork | 0.338 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT, RIGID BODY REFINEMENT |
| Starting model (for MR) | 1ojt |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.290 |
| High resolution limit [Å] | 4.180 | 4.180 |
| Rmerge | 0.055 | 0.060 |
| Total number of observations | 44222 * | |
| Number of reflections | 6008 | |
| <I/σ(I)> | 5.2 | 10.7 |
| Completeness [%] | 98.2 | 78 |
| Redundancy | 7.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | Li De La Sierra, I., (1994) J.Mol.Biol., 235, 1154. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.02 (M) | |
| 3 | 1 | drop | EDTA | 0.006 (M) | |
| 4 | 1 | drop | 0.15 (M) | ||
| 5 | 1 | reservoir | potassium phosphate | 0.1 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 2 (M) |
