1BHT
NK1 FRAGMENT OF HUMAN HEPATOCYTE GROWTH FACTOR
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1994-01 |
Detector | FUJI |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 88.320, 88.320, 117.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.24700 |
Structure solution method | MIR |
RMSD bond length | 0.013 |
RMSD bond angle | 2.051 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.090 | |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.066 | 0.170 |
Number of reflections | 1807 | |
<I/σ(I)> | 11.7 | |
Completeness [%] | 94.0 | 87 |
Redundancy | 5.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 | 40% PEG 3400, 0.1M HEPES PH 7.5, 0.3M AMMONIUM SULFATE. PROTEIN CONCENTRATION OF 3MG/ML |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3 (mg/ml) | |
2 | 1 | reservoir | PEG3400 | 20 (%) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | |
4 | 1 | reservoir | ammonium sulfate | 0.3 (M) |