1BHS

HUMAN ESTROGENIC 17BETA-HYDROXYSTEROID DEHYDROGENASE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-11-16
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 1 2 1
Unit cell lengths	123.560, 45.050, 61.330
Unit cell angles	90.00, 99.02, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.185
Rwork	0.185
R-free	0.22900
RMSD bond length	0.010
RMSD bond angle	1.680 *
Data reduction software	RIGAKU
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.064
Total number of observations	43960 *
Number of reflections	14079
Completeness [%]	81.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		0.075 (M)
3	1	drop	HEPES	0.05 (M)
4	1	drop	PEG4000	14 (%)
5	1	drop	glycerol	10 (%)
6	1	reservoir		0.15 (M)
7	1	reservoir	HEPES	0.1 (M)
8	1	reservoir	PEG4000	27 (%)