1BHM
RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 108.800, 81.900, 68.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.189 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.800 |
Refinement software | TNT |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.118 * |
Total number of observations | 103881 * |
Number of reflections | 30078 |
Completeness [%] | 94.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | Strzelecka, T., (1994) J. Mol. Biol, 239, 430. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
10 | 1 | 2 | WATER | ||
11 | 1 | 2 | PEG 8000 | ||
12 | 1 | 2 | GLYCEROL | ||
13 | 1 | 2 | KCL | ||
2 | 1 | 1 | GLYCEROL | ||
3 | 1 | 1 | PEG 8000 | ||
4 | 1 | 1 | K PHOSPHATE | ||
5 | 1 | 1 | DTT | ||
6 | 1 | 1 | KCL | ||
7 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
8 | 1 | 1 | EDTA | ||
9 | 1 | 1 | NACL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | BamHI | 0.23 (mM) | |
10 | 1 | drop | PEG8000 | 12 (%) | |
11 | 1 | drop | glycerol | 2 (%) | |
12 | 1 | reservoir | PEG8000 | 12 (%) | |
13 | 1 | reservoir | 0.15 (M) | ||
14 | 1 | reservoir | glycerol | 5 (%) | |
2 | 1 | drop | DNA | 0.46 (mM) | |
3 | 1 | drop | glycerol | 3 (%) | |
4 | 1 | drop | 6.1 (mM) | ||
5 | 1 | drop | 0.3 (mM) | ||
6 | 1 | drop | 0.15 (M) | ||
7 | 1 | drop | Tris-HCl | 3.9 (mM) | |
8 | 1 | drop | 0.4 (mM) | ||
9 | 1 | drop | 3.9 (mM) |