1BHH
FREE P56LCK SH2 DOMAIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12-10 |
| Detector | RIGAKU |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.800, 58.800, 110.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.000 |
| Data reduction software | RIGAKU |
| Data scaling software | RIGAKU |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.060 |
| Total number of observations | 42626 * |
| Number of reflections | 13477 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | drop | 1.8 (%) | ||
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | pH4.5 |
| 4 | 1 | reservoir | PEG4000 | 30 (%) | |
| 5 | 1 | reservoir | ammonium acetate | 200 (mM) |
