1BHD
SECOND CALPONIN HOMOLOGY DOMAIN FROM UTROPHIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08-25 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.920, 32.210, 65.360 |
Unit cell angles | 90.00, 116.25, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.185 * |
Rwork | 0.185 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 1AA2 |
RMSD bond length | 0.015 |
RMSD bond angle | 0.036 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 2.100 |
High resolution limit [Å] | 2.000 | 1.990 |
Rmerge | 0.032 | 0.125 |
Total number of observations | 46728 * | |
Number of reflections | 16459 * | 2296 * |
<I/σ(I)> | 10.8 | 5.9 |
Completeness [%] | 99.5 | 98.9 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.6 | 20 * | pH 7.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 18 (%) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
4 | 1 | reservoir | HEPES | 0.1 (M) |