1BH9

HTAFII18/HTAFII28 HETERODIMER CRYSTAL STRUCTURE WITH BOUND PCMBS

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM02
Synchrotron site	ESRF
Beamline	BM02
Temperature [K]	100
Detector technology	CCD
Collection date	1998-01
Spacegroup name	P 21 21 21
Unit cell lengths	45.800, 48.400, 70.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.205
Rwork	0.205
R-free	0.25700
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.480
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	CNS (0.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.600	2.600
Rmerge	0.088 *	0.173 *
Total number of observations	35105 *
Number of reflections	5119
<I/σ(I)>	26.8	15.3
Completeness [%]	99.3	99.7
Redundancy	6.9	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.9 *		drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8-15 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	PEG4000	10 (%)
4	1	reservoir	ammonium sulfate	10 (%)
5	1	reservoir	HEPES	100 (mM)