1BH8

HTAFII18/HTAFII28 HETERODIMER CRYSTAL STRUCTURE

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM02
Synchrotron site	ESRF
Beamline	BM02
Temperature [K]	100
Detector technology	CCD
Collection date	1998-01
Spacegroup name	P 21 21 21
Unit cell lengths	45.400, 48.400, 77.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.000
R-factor	0.199
Rwork	0.199
R-free	0.28200
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.450
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	CNS (0.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.039 *	0.115 *
Total number of observations	9480 *
Number of reflections	3376
<I/σ(I)>	21.6	12.8
Completeness [%]	82.0	78.1
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.9 *	4 *	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8-15 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	PEG4000	10 (%)
4	1	reservoir	ammonium sulfate	100 (mM)
5	1	reservoir	HEPES