1BGI
ORTHORHOMBIC LYSOZYME CRYSTALLIZED AT HIGH TEMPERATURE (310K)
Experimental procedure
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-06 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.440, 73.730, 30.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lzt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.200 * |
| Phasing software | AUTOMR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 11992 |
| Completeness [%] | 82.6 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch method | 4.7 | 310 | CRYSTALLIZATION WAS PERFORMED BY THE BATCH METHOD AT 310 K. THE SAME WAY AS DESCRIBED BY JOLLES AND BERTHOU (1972)., pH 4.7, batch method |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | acetate | 0.2 (M) | 0.625ml |
| 2 | 1 | 1 | water | 1.875ml | |
| 3 | 1 | 1 | 100 (mg/ml) | 7.5ml |
