1BGG

GLUCOSIDASE A FROM BACILLUS POLYMYXA COMPLEXED WITH GLUCONATE

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE
Synchrotron site	LURE
Temperature [K]	176
Detector technology	IMAGE PLATE
Collection date	1996-10
Detector	MARRESEARCH
Spacegroup name	P 42 21 2
Unit cell lengths	205.850, 205.850, 155.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.2
Rwork	0.200
R-free	0.25000
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1bga
RMSD bond length	0.009
RMSD bond angle	24.010 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.400	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.092 *	0.320
Total number of observations	840221 *
Number of reflections	146539
<I/σ(I)>	6.2	2
Completeness [%]	99.4	97.2
Redundancy	5.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	co-crystallization	8.3		COMPLEX WAS OBTAINED BY CO-CRYSTALLIZATION, 5 MICRO-L BGLA (14 MG/ML) / 5 MICRO-L 10MM LIGAND / 5 MICRO-L PP 1.3M, PH 8.3, co-crystallization

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	inhibitor
2	1	drop	phosphate	1.3 (M)