1BGE
CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.200, 35.900, 91.000 |
| Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.500 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.046 * |
| Number of reflections | 15536 * |
| Completeness [%] | 90.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.54 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | canine G-CSF solution | 18.0 (mg/ml) | |
| 2 | 1 | drop | ethanol | 5.85 (%) | |
| 3 | 1 | drop | HEPES | 50 (mM) | adjusted to pH6.54 by NaOH |
| 4 | 1 | drop | ammonium sulfate | 255 (mM) | |
| 5 | 1 | reservoir | ethanol | 5.85 (%) | |
| 6 | 1 | reservoir | HEPES | 50 (mM) | adjusted to pH6.54 by NaOH |
| 7 | 1 | reservoir | ammonium sulfate | 255 (mM) |
