1BGC
CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.000, 53.900, 57.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 1.700 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.590 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 * |
| Rmerge | 0.055 * |
| Total number of observations | 74290 * |
| Number of reflections | 15100 * |
| Completeness [%] | 93.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 3.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | bovine G-CSF solution | 9 (mg/ml) | |
| 2 | 1 | drop | MES | 50 (mM) | adjusted to pH5.5 with NaOH |
| 3 | 1 | reservoir | MES | 50 (mM) | adjusted to pH5.5 with NaOH |
