1BG7

LOCALIZED UNFOLDING AT THE JUNCTION OF THREE FERRITIN SUBUNITS. A MECHANISM FOR IRON RELEASE?

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	120
Detector technology	CCD AREA DETECTOR
Collection date	1998-02
Detector	SIEMENS
Spacegroup name	F 4 3 2
Unit cell lengths	182.800, 182.800, 182.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.850
R-factor	0.207
Rwork	0.207
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fha
RMSD bond length	0.012
RMSD bond angle	20.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.890
High resolution limit [Å]	1.800	1.810
Rmerge	0.078	0.107
Total number of observations	1179393 *
Number of reflections	1179393
<I/σ(I)>	37	0.8
Completeness [%]	99.2	98.1
Redundancy	60	50

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	MPD	15 (%)
3	1	reservoir		0.02 (M)
4	1	reservoir	sodium acetate	0.1 (M)