1BG7
LOCALIZED UNFOLDING AT THE JUNCTION OF THREE FERRITIN SUBUNITS. A MECHANISM FOR IRON RELEASE?
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 120 |
| Detector technology | CCD AREA DETECTOR |
| Collection date | 1998-02 |
| Detector | SIEMENS |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.800, 182.800, 182.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.850 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fha |
| RMSD bond length | 0.012 |
| RMSD bond angle | 20.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.890 | |
| High resolution limit [Å] | 1.800 | 1.810 |
| Rmerge | 0.078 | 0.107 |
| Total number of observations | 1179393 * | |
| Number of reflections | 1179393 | |
| <I/σ(I)> | 37 | 0.8 |
| Completeness [%] | 99.2 | 98.1 |
| Redundancy | 60 | 50 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 15 (%) | |
| 3 | 1 | reservoir | 0.02 (M) | ||
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) |
