1BG2

HUMAN UBIQUITOUS KINESIN MOTOR DOMAIN

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-06
Detector	MAR scanner 180 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	48.540, 67.940, 112.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.2159
Rwork	0.216
R-free	0.28800
Structure solution method	MIR
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.840
High resolution limit [Å]	1.800	1.770
Number of reflections	30582
<I/σ(I)>	12.5	2.1
Completeness [%]	82.4	49.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		5 MG/ML PROTEIN, 50 MM NA ACETATE PH 4.6, 75 MM KCL, 3.5% PEG 4K, 2.5 MM ATP, 10 MM MGCL2