1BG1
TRANSCRIPTION FACTOR STAT3B/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 |
| Unit cell lengths | 174.000, 174.000, 79.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.246 * |
| Rwork | 0.246 |
| R-free | 0.30100 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 21.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.053 | 0.300 |
| Total number of observations | 273455 * | |
| Number of reflections | 56005 | |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 4.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | protein-DNA solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.074 (mM) | |
| 10 | 1 | reservoir | MES | 50 (mM) | |
| 11 | 1 | reservoir | ammonium acetate | 0.1 (M) | |
| 12 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | drop | PMSF | 0.5 (mM) | |
| 7 | 1 | drop | DNA duplex | ||
| 8 | 1 | reservoir | 0.1-0.4 (M) | ||
| 9 | 1 | reservoir | 5 (mM) |
