1BFS
STRUCTURE OF NF-KB P50 HOMODIMER BOUND TO A KB SITE
Experimental procedure
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-25 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.330, 62.330, 65.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.200 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MONOMER A OF DIMERIZATION DOMAINS OF P50RHR/DNA COMPLEX |
RMSD bond length | 0.010 |
RMSD bond angle | 26.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.049 | 0.270 |
Number of reflections | 7896 | |
<I/σ(I)> | 13.2 | 4.1 |
Completeness [%] | 98.0 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | cacodylate | 0.08 (M) | |
2 | 1 | reservoir | ammonium sulfate | 0.16 (M) | |
3 | 1 | reservoir | PEG8000 | 30 (%) | |
4 | 1 | drop | cacodylate | 0.04 (M) | |
5 | 1 | drop | ammonium sulfate | 0.08 (M) | |
6 | 1 | drop | PEG8000 | 15 (%) | |
7 | 1 | drop | p50dd | 7 (mg/ml) |