1BFP

BLUE VARIANT OF GREEN FLUORESCENT PROTEIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1996-09-19
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	52.010, 63.090, 69.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.181 *
Rwork	0.181
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
RMSD bond length	0.010
RMSD bond angle	1.970
Data reduction software	DATAC
Data scaling software	SCALE1
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.260
High resolution limit [Å]	2.100	2.100
Rmerge	0.041	0.140
Total number of observations	31786 *
Number of reflections	12044
<I/σ(I)>	12.7	2.24
Completeness [%]	87.0	65 *
Redundancy	2.64	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *	PROTEIN WAS CRYSTALLIZED AT 4 DEGC FROM 100MM SODIUM ACETATE PH 4.5 AND 10-12% PEG3400., temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	reservoir	sodium acetate	100 (mM)
4	1	reservoir	PEG3400	10-12 (%)