1BFP
BLUE VARIANT OF GREEN FLUORESCENT PROTEIN
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 1996-09-19 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.010, 63.090, 69.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.181 * |
Rwork | 0.181 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ema |
RMSD bond length | 0.010 |
RMSD bond angle | 1.970 |
Data reduction software | DATAC |
Data scaling software | SCALE1 |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.260 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.041 | 0.140 |
Total number of observations | 31786 * | |
Number of reflections | 12044 | |
<I/σ(I)> | 12.7 | 2.24 |
Completeness [%] | 87.0 | 65 * |
Redundancy | 2.64 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 4 * | PROTEIN WAS CRYSTALLIZED AT 4 DEGC FROM 100MM SODIUM ACETATE PH 4.5 AND 10-12% PEG3400., temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | reservoir | sodium acetate | 100 (mM) | |
4 | 1 | reservoir | PEG3400 | 10-12 (%) |